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| Zhang,J.P.; Li,N.; Bai,W.Z.; Qiu,X.C.; Ma,B.A.; Zhou,Y.; Fan,Q.Y.; Shan,L.Q.. |
| Notch signaling plays a vital role in tumorigenicity and tumor progression by regulating proliferation, invasion, and the tumor microenvironment. Previous research by our group indicated that Notch ligand Delta-like 1 (Dll1) is involved in angiogenesis in melanoma, and we noticed that it took a longer time to trypsinize Dll1-expressing B16 melanoma cells than the control cells. In this article, we extended our study to investigate the effects of Dll1 on tumor cell adhesion and metastasis. Dll1 overexpression activated Notch signaling in B16 tumor cells and significantly enhanced the adhering capacity of B16 tumor cells both in vitro and in vivo. B16-Dll1 cells also had a higher metastatic potential than their counterpart in the mouse model of lung... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Delta-like 1; Notch signaling; Melanoma; Metastasis; Adhesion; N-cadherin. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400299 |
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| Ren,H.J.; Zhang,C.L.; Liu,R.D.; Li,N.; Li,X.G.; Xue,H.K.; Guo,Y.; Wang,Z.Q.; Cui,J.; Ming,L.. |
| The epithelium is a highly dynamic system, which plays a crucial role in the homeostasis of the intestinal tract. However, studies on the physiological and pathophysiological functions of intestinal epithelial cells (IECs) have been hampered due to lack of normal epithelial cell models. In the present study, we established a reproducible method for primary culture of mouse IECs, which were isolated from the viable small intestinal crypts of murine fetuses (on embryonic day 19), using type I collagenase and hyaluronidase in a short span of time (≤20 min). With this method, continuously growing mouse IECs, which can be subcultured over a number of passages, were obtained. The obtained cell lines formed a tight cobblestone-like arrangement, displayed long and... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Intestinal epithelial cells; Crypt; Isolation; Primary culture; Collagenase I; Hyaluronidase. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500604 |
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| Zhao,L.; Li,N.; Yu,J.K.; Tang,H.T.; Li,Y.L.; He,M.; Yu,Z.J.; Bai,X.F.; Zheng,Z.H.; Wang,E.H.; Wei,M.J.. |
| Fanconi anemia complementation group F protein (FANCF) is a key factor, which maintains the function of FA/BRCA, a DNA damage response pathway. However, the functional role of FANCF in breast cancer has not been elucidated. We performed a specific FANCF-shRNA knockdown of endogenous FANCF in vitro. Cell viability was measured with a CCK-8 assay. DNA damage was assessed with an alkaline comet assay. Apoptosis, cell cycle, and drug accumulation were measured by flow cytometry. The expression levels of protein were determined by Western blot using specific antibodies. Based on these results, we used cell migration and invasion assays to demonstrate a crucial role for FANCF in those processes. FANCF shRNA effectively inhibited expression of FANCF. We found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fanconi anemia complementation group F protein; Breast neoplasms; Tumor cell line. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100024 |
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| Li,N.; Wu,H.M.; Hang,F.; Zhang,Y.S.; Li,M.J.. |
| Immunological mechanisms have been proposed to underlie the pathogenesis of recurrent spontaneous abortion (RSA). Vitamin D has a potent immunomodulatory effect, which may affect pregnancy outcome. The objective of this study was to investigate 25-hydroxyvitamin D [25(OH) D] concentration and vitamin D receptor (VDR) expression in the decidual tissues of RSA patients. Thirty women with RSA (RSA group) and thirty women undergoing elective abortion (control group) were recruited during 2016 from gynecology outpatient clinics. We measured 25(OH) D, interleukin (IL)-17, IL-23, transforming growth factor β (TGF-β), VDR and 1-α-hydroxylase (CYP27B1) in decidual tissues collected during the abortion procedure. In the RSA group, 25(OH) D and TGF-β were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Recurrent spontaneous abortion (RSA); Treg/Th17 cells; Maternal-fetal interface; 25(OH) D; Vitamin D metabolism. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100606 |
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